Transfer Pipettes
A look at what is currently available on eBay
 NEW!! 500 pcs Plastic Transfer Pipettes Droppers!! US $12.00 
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 50 Plastic Transfer Pipettes Droppers Graduated 1 ml US $4.88
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 Transfer Pipette Graduated Dropper US $2.99
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 500 Plastic Transfer Pipettes Droppers Graduated 3 ml US $18.88
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 5 pc- graduated 3 ml (3 c.c.) Transfer Pipette pipet Dropper US $1.75
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 100x Plastic Transfer Pipettes Dropper Polyethylene 3ml US $6.00
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You have three pipettes available to deliver 1000 μl as part of an assay.?
You have three pipettes available to deliver 1000 μl as part of an assay. Estimate
the error in your volume transfer if you
(1) Deliver two 500 μl aliquots with a 500 μl pipetter with an estimated 0.5%
error per addition
(2) Deliver ten 100 μl aliquots with a 100 μl pipetter with an estimated 0.5% error
per addition
(3) Deliver fifty 20 μl aliquots with a 20 μl pipetter with an estimated 0.5% error
per addition
Do you think all pipettes, regardless of size, have the same error per addition? How do
you think reality would impact the above exercise –what approach would yield the largest
Using an error volume approach:
500 μ pipette will add: 500/100 x .5 = 2.5 μl per aliquot
or : 2.5 x 2 = 5 μl total
100 μ pipette will add: 100/100 x .5 = 0.5 μl per aliquot
or : 0.5 x 10 = 5 μl total
20 μ pipette will add: 20/100 x .5 = 0.1 μl per aliquot
or :0.1 x 50 = 5 μl total
so in theory their total errors the same no matter what you use. In the real world errors go up the smaller the volume measures so you would be better off with 500 μl pipette. It is also easier to miss count larger number of additions.
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December 6th, 2005 in
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