Pcr System
A look at what is currently available on eBay
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 PE perkin Elmer cycler PCR system GeneAmp 2400 gene amp US $495.00
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 Perkin Elmer GeneAmp PCR System 9600 US $499.00
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 Perkin-Elmer GeneAmp PCR System 2400 Part# N8030001! US $600.00
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 PERKIN ELMER 9600 PCR SYSTEM GENEAMP THERMAL CYCLER! US $600.00
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 Applied Biosystems GeneAmp PCR System 2700 - P/N: 4322620 US $595.00
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 Perkin Elmer GeneAmp PCR System 2400 US $400.00
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 PERKIN ELMER GENEAMP PCR SYSTEM 9600 W/ MANUAL US $299.99
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 PERKIN ELMER GENEAMP PCR SYSTEM 2400 THERMAL CYCLER US $349.99
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 Perkin Elmer GeneAmp PCR 9600 System US $1,144.00
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 Dual 384-Well GeneAmp PCR System 9700 IN GOOD WORKING CONDITION US $1,300.00
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 ABI GENEAMP PCR SYSTEM 2700 With Manual US $2,999.00
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 PERKIN ELMER GENEAMP PCR SYSTEM 2400 THERMAL CYCLER N8030001 US $399.99
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 ERICOMP EZ POWERBLOCK II SYSTEM PCR THERMAL CYCLER US $1,002.00
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uracil glycosylase?
Has anyone used Uracil glycosylase system for preventing contamination of PCRs?Do we need to keep the PCR at 72 degrees for ever?I usually have a 5 minute extension at 72 degrees at the end,so IS IT ok?Also will UNG get destroyed at this temp?What happens if I need to run a gel?
Without looking at your protocol, it would be difficult to tell, but perhaps this short paper will address some of your concerns:
J Microbiol Methods. 1999 Feb;35(1):73-6.
If not, they reference other protocols that have alternative methods to inactivating UNG at the end of the cycle.
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August 11th, 2005 in
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