Objective Lens
A look at what is currently available on eBay
 Leitz Wetzlar microscope objective lens 3.5 /0.10 170/- US $20.00 
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 Leitz Wetzlar microscope objective lens 10/0.25 170/- US $20.00
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 Leitz Wetzlar microscope objective lens 40/0.65 170/0.17 US $20.00
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2) Why should the coarse-adjustment knob not be used with a high-power objective lens?
Why should the coarse-adjustment knob not be used with a high-power objective lens?
With the standard light microscope, the coarse adjustment knob is routinely used with low-power objective lens because they focus further away from the slide, specimen, and cover-slip. So, you have a range there for making coarse adjustment to the focus. However, when you are using the high-power objective lens, the focus is already very close to the specimen and cover-slip. A coarse adjustment there may accidentally drive the objective lens housing into the cover-slip, specimen and slide, damaging them. Always, when using the high-power objective lens, visually bring the objective lens into "approximate" focus position, then use the "fine" adjustment knob to focus for the best image.
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