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Is there an spectrophotometer that measures optical density, pH and tempreture for a 96 well plate?
Can anyone tell me if the Molecular Devices Spectra Max M2 instrument will measure pH in a 96 well plate? I know it measures absorbance (optical density) and is temperature controlled, but does it measure temperature and pH in individual wells? Is there any other instrument that does this?
The Molecular Devices Spectra Max M2 will not measure pH in a 96 well plate and does not measure temperature in each individual well, just the plate itself. However, if you want to measure pH in each well you can still use the plate reader. Add BCECF-AM (its available at Sigma) to each well and measure with an excitation of 485 nm and emission 538 nm. You will also need to make a standard curve of known pH in order to determine what OD corresponds to the pH.
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