Fluorescent Microscope
A look at what is currently available on eBay
 12W RING BULB FOR MICROSCOPE FLUORESCENT RING LIGHT US $9.95 
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 Adjustable 10 Watt Fluorescent Microscope Ring Light US $49.99
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 FLUORESCENT RING LIGHT 4 STEREO MICROSCOPE + SPARE BULB US $24.50
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Lens question mircoscope?
Herpes viruses are labeled w/ a fluorescent dye that emits light of wavelength 450 nm. If the viruses are viewed in a microscope with aperture of teh objective lens of 1.5mm what is the minimum distance apart two virus can be and still be resolved.
a) 0.11 micrometers
b) 0.22 micrometers
c) 0.18 micrometers
d) 1.3 micrometers
e) none of the above
The Rayleigh resolution limit is an angle θ.
sinθ = 1.22λ/D; in this case, with D = 1500000 nm and λ = 450 nm, θ ~ sinθ = 0.000366 rad. Since the focal distance isn't given, the linear resolution cannot be calculated.
However, the ref. gives a best possible value for a microscope of 187 nm, based on practical limits on objective lenses.
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February 24th, 2008 in
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