Electrophoresis Power
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agarose gel electrophoresis?
whats the purpose of
agarose gel
TBE buffer
power supply
DNA samples
and DNA stain????
Agarose gel is a porous gel that allows pieces of stuff (in this case, DNA) to pass through it.
TPE buffer is the liquid used, it's a physiological pH.
The DNA samples + stain are loaded into the wells at one end of the gel. A power supply is added, where the positive electrode is at the opposite end to where the DNA has been loaded. Since DNA is negatively charged, it will move through the gel toward the positive end. Smaller fragments move easier and quicker, while larger fragments move slower.
When the power is shut off, the gel is photographed with a special UV camera that recognizes the dye. The smaller fragments will be at the bottom (faster moving), and the bigger on the top (slower moving).
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EC 4000p 4000 Volts Programmable Electrophoresis Power Supply,
January 10th, 2007 in
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