Absorbance Detector
A look at what is currently available on eBay
 Rainin Dynamax HPLC system DAD pump SD-200 Absorbance Detector UV preparative US $3,455.00 
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 DIONEX AD20 ABSORBANCE DETECTOR US $950.00
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 BECKMAN 160 ABSORBANCE DETECTOR US $239.99
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 ISCO Model 1840 Absorbance Detector US $210.00
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 ISCO UA-5 Absorbance Fluoresence Detector US $149.99
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 Waters 486 Tunable Absorbance Detector US $2,495.00
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 Applied Biosystems 757 Absorbance Detector US $199.00
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 DUPONT INDUSTRIES 850 ABSORBANCE DETECTOR US $275.00
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 BECKMAN 160 ABSORBANCE DETECTOR 160 US $350.00
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 Isco V4 Absorbance Detector US $395.00
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 RAININ DYNAX UV-DII ABSORBANCE DETECTOR NICE US $499.99
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 Waters 484 Tunable Absorbance Detector US $995.00
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 DU Pont 860 Absorbance Detector US $280.00
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 Waters M484 HPLC Turnable Absorbance Detector 484 US $179.99
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 Applied Bio System Absorbance Detector Model 757 US $239.99
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 WATERS 486 TUNABLE ABSORBANCE DETECTOR WORKING US $750.00
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 APPLIED BIOSYSTEMS 783 PROGRAMMABLE ABSORBANCE DETECTOR US $649.99
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 Waters 486 Tunable Absorbance Detector US $999.99
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Amount of light decreases,absorbance increases? ?
Two peaks observed in a spectrum are the result of absorption of light at two different wavelengths (around 250 nm and around 400 nm). Because of the absorption of light by the metal ion, the amount of light getting to the detector decreases, yet the absorbances are shown in the graph as increases (shown as positive peaks). Please explain?
This one's real simple. What actually gets measured is the _transmittance_, which is the ratio of the amount of light that "makes it through" the sample, to the amount of light that entered it. (Or the amount of light that reaches the detector with the sample present, versus the amount of light that reaches the detector when the sample is NOT present.) As you would expect, the percent transmittance (%T) _decreases_ when the sample gets darker.
Absorbance is the _negative_ log of %T (A = -log(%T)), so a large %T means a small A, and vice versa. And as the amount of light decreases, the absorbance increases.
(You can think of absorbance as a measure of the light that got "soaked up" - absorbed - by the sample, if that helps.)
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January 21st, 2007 in
Uncategorized | tags: absorbance detector, absorbance detector capillary, absorbance detector diagram, absorbance detector flow cell, absorbance detector hplc, cytometry, detector, flow, research, uv, uv absorbance detector